首页> 外文OA文献 >Identifikasi 26 Isolat Bakteri Endofitik Dan Filosfer Padi Dengan Analisis Sekuen16s Rdna [Identification of 26 Endophytic and Phyllosphere Bacteria Isolated From Rice by 16s Rdna Sequence Analyses]
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Identifikasi 26 Isolat Bakteri Endofitik Dan Filosfer Padi Dengan Analisis Sekuen16s Rdna [Identification of 26 Endophytic and Phyllosphere Bacteria Isolated From Rice by 16s Rdna Sequence Analyses]

机译:用16s Rdna序列分析鉴定26种内生细菌分离株和水稻菌丝体[通过16s Rdna序列分析鉴定水稻中26种内生细菌和离体的细菌]

摘要

The research was subjected to identify 26 endophytic and phyllosphere bacteria isolated from 4 rice varieties using sequencing method of 16S rDNA.The sequences were then aligned with reference sequence from Gen-Bank data library by BLAST program from NCBI to find the most related identity of the isolates analysed.The sequencing analysis revealed that some isolates have a high identity to Staphylococcus and Serratia (5 isolates each), Bacillus (4 isolates), Microbacteria (3 isolates), Pseudomonas (2isolates) and Klebsiella, Acidovorak, Bulkholderiaceae, Agrobacterium and Shewanella (1 isolate each). No specific isolate dominated in both endophytic and phyllosphere group, nor host-specific isolate found in the four rice varieties.
机译:该研究采用16S rDNA测序方法鉴定了从4个水稻品种中分离的26种内生和叶系细菌,然后通过NCBI的BLAST程序将其序列与Gen-Bank数据库的参考序列进行比对,以找到与水稻最相关的身份。测序分析表明,某些分离株与葡萄球菌和沙雷氏菌(各5个分离株),芽孢杆菌(4个分离株),微细菌(3个分离株),假单胞菌(2个分离株)和克雷伯菌,酸杆菌,酸菌,大果菌科,农杆菌和希瓦氏菌具有高度同一性(各隔离1个)。内生和叶环组均未发现任何特异分离株,在这四个水稻品种中均未发现宿主特异分离株。

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